Research
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Toxicology Resource Laboratory

A wide variety of assay capabilities are available to the research investigator. Qualitative and quantitative determination of the concentration of heavy metals, toxins, pesticides, that may constitute a health risk to animals and humans. Metabolism studies.

A. Instrumentation

  • Atomic Absorption Spectrophotometer (AA) with Graphite Furnace and Hydride Generation Accessories
  • High Pressure Liquid Chromatography (HPLC) with UV-visible, electrochemical and fluorescence detection.
  • Gas Chromatography (GC) with flame ionization detection (GC-FID) and mass-spectrometry detection (GC-MS).
  • UV-Visible Spectrophotometer
  • Microplate reader
  • Fluorescence Spectrophotometer
  • Cytofluor 2300 Fluorescence Measurement System

B. Technical Expertise

  • Enzyme assays: Acetylcholinesterase, Carboxylesterases, Ceruloplasmin, Glutathione peroxidase, Glutathione-S-transferase, Metabolizing enzymes (i.e., Aniline Hydroxylase, Cytochrome P450, O-Demethylase), Protein analysis, Superoxide dismutase.
  • Analysis of metals and minerals in agricultural, food, and biochemical samples: Arsenic, Cadmium, Calcium, Chromium, Copper, Iron, Lead, Magnesium, Molybdenum, Selenium, Sodium, Zinc.
  • Mycotoxins Analyses: Aflatoxins, Fumonisin, Vomitoxin, Tremorgens (Roquefortine C, Penitrem A).
  • Drugs/Xenobiotics Analyses: Anticoagulant rodenticides (Brodifacoum, Warfarin, Bromadiolone, Difenacoum, Difethialone, Diphacinone, Chlorophacinone), Ethylene glycol, Ionophores screen (Lasalocid, Monensin, Narasin, Salinomycin), Strychnine, Urea, Insecticides, barbiturates (pentobarbital, phenobarbital).
  • Miscellaneous drugs/xenobiotics (pentobarbital, )
  • Serum, liver and feed Vitamin A & E and b-carotene.
  • Miscellaneous Analyses: Cyanide, Nitrates, Met-hemoglobin.
  • Fluorescence Spectrophotometry: Intracellular ion concentration (i.e., Ca), Membrane receptors (i.e., acetylcholine, dopamine), Cytotoxicity analysis of naturally fluorescent compounds.
  • Metabolism studies: catecholamines, vitamin C, isoprostanes

C. Using the Toxicology Research Laboratory

Persons wishing to use the toxicology laboratory for research should contact Dr. Geraldine Magnin-Bissel, laboratory manager, at 540-231-8689 or ).

Limited technical support precludes extensive assay research and development by technicians. Technical support is available for assays which have been developed and proven.

Costs depend upon the instrument used and the preparation procedure needed for the assay. The charges include the cost of supplies used for the assay (solvent, reagents, columns) as well as the cost of the service contract needed to maintain the instrumentation. Researchers are expected to purchase all reagents and supplies necessary for non-routine analytical procedures. In addition, researchers are charged instrument service fees to help defray service contract costs.

D. Projects Currently in Progress

  • Analysis of Eastern Tent Caterpillars and their feces for the presence of tremorgens as possible agents responsible for the Mare Reproductive Loss Syndrome.
  • In vitro systems for assessment of neurotoxicity and cytotoxicity and transfer of toxicants to the nervous system.
  • Pesticide screens In vitro using cell culture systems.
  • Species differences in toxic effects of anti-esterase pesticides.

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