Lijuan Yuan, MS, PhD is an Associate Professor of Virology and Immunology in the VA-MD Vet Med’s Department of Biomedical Sciences & Pathobiology.
The mission of Dr. Yuan’s laboratory is to study the interactions among enteric viruses, probiotics, and the host immune system.
The lab's research activities are focused on:
- Pathogenesis and innate and adaptive immune responses induced by enteric viruses (i.e. norovirus and rotavirus) in gnotobiotic pig models of human enteric virus infection and diseases
- Mechanism of immune modulation by probiotics
- The development of safer and more effective vaccines against viral gastroenteritis
Some virology and immunology techniques used in the Yuan lab's research:
- Rotavirus particles in fecal samples detected by immune electron microscopy.
- Rotavirus replication in porcine jejunum epithelial cells detected by immunofluorescence staining
- Cytopathological changes in porcine small intestinal epithelium caused by human norovirus infection observed under transmission electron microscopy.
- Immune cell analysis and sorting by flow cytometry.
- Antibody secreting cells detected by ELISPOT assay.
- Real-time PCR melting curve for detection and quantitation of human norovirus.