Meng Laboratory: Research Interests

Dr. Meng's lab studies the molecular mechanisms of viral replication and pathogenesis and developing effective vaccines against emerging and zoonotic viral diseases. Currently the Meng lab mainly studies hepatitis E virus (human, swine, avian, and rabbit HEVs), and porcine reproductive and respiratory syndrome virus (PRRSV). The Meng lab also studies porcine circovirus type 2 (PCV2), Torque teno sus virus (TTSuV), porcine epidemic diarrhea virus (PEDV), and mammalian orthoreovirus type 3 (MRV3).

I: Hepatitis E virus (HEV)

HEV, the causative agent of human hepatitis E, belongs to the family Hepeviridae. The virus causes both acute and chronic hepatitis. In 1997, we discovered and characterized the first animal strain of HEV, swine HEV, from a pig in the United States. We showed that swine HEV can cross species barriers and infect non-human primates. In 2001, our group discovered yet another animal strain of HEV, avian HEV, from chickens with Hepatitis-Splenomegaly syndrome in the United States. Hepatitis E is now a recognized zoonotic disease. Currently, our research efforts focus on studying the structural and functional relationship of HEV genes, and understanding the molecular mechanisms of HEV cross species infection, neuroinvasion, chronic infection, and fulminant hepatitis during pregnancy.

Representative publications:

  • Meng X.J., R.H. Purcell, P.G. Halbur, J.R. Lehman, D.M. Webb, T.S. Tsareva, J.S. Haynes, B.J. Thacker, and S.U. Emerson (1997). A novel virus in swine is closely related to the human hepatitis E virus. Proceedings of the National Academy of Sciences USA 94:9860-9865. ( Abstract)
  • Meng X.J., P.G. Halbur, M. Shapiro, S. Govindarajan, J.D. Bruna, I. K. Mushahwar, R.H. Purcell, and S.U. Emerson (1998). Genetic and experimental evidence for cross-species infection by the swine hepatitis E virus. Journal of Virology. 72:9714-9721. ( Abstract)
  • Kabrane-Lazizi, Y., X.J. Meng, R.H. Purcell, and S.U. Emerson. Evidence that the genomic RNA of hepatitis E virus is capped (1999). Journal of Virology. 73:8848-8850. ( Abstract)
  • Emerson SU, Zhang M, Meng X.J., St. Clair M, Nguyen H, Huang Y, and Purcell RH (2001). Recombinant hepatitis E virus genomes infectious for primates: importance of capping and discovery of a cis-reactive element. Proceedings of the National Academy of Sciences USA 98:15270-15275. ( Abstract)
  • Haqshenas, G., H.L. Shivaprasad, P. Woolcock, D. Read, and X.J. Meng (2001). Genetic identification and characterization of a novel virus related to human hepatitis E virus from chickens with hepatitis-spleenomegaly syndrome. Journal of General Virology. 82:2449-2462. ( Abstract)
  • Huang Y.W., G. Haqshenas, C. Kasorndorkbua, P.G. Halbur, S.U. Emerson, and X.J. Meng (2005). Capped RNA transcripts of full-length cDNA clones of swine hepatitis E virus are replication-competent when transfected into Huh7 cells and infectious when intrahepatically inoculated into pigs. Journal of Virology. 79:1552-1558. ( Abstract)
  • Billam P., F.F. Huang, Z.F. Sun, F.W. Pierson, R.B. Duncan, F. Elvinger, D.K. Guenette, T.E. Toth, and X.J. Meng (2005). Systematic pathogenesis and replication of avian hepatitis E virus in specific-pathogen-free adult chickens. Journal of Virology. 79:3429-3437. ( Abstract)
  • Huang YW, Oprissnig T, Halbur PG, and Meng XJ (2007). Initiation at the third in-frame AUG codon of open reading frame 3 of the hepatitis E virus is essential for viral infectivity in vivo. Journal of Virology. 81:3018-3026. ( Abstract)
  • Pudupakam RS, Huang YW, Opriessnig T, Halbur PG, Pierson FW, X.J. Meng (2009). Deletions of the hypervariable region (HVR) in open reading frame 1 of hepatitis E virus do not abolish virus infectivity: evidence for attenuation of HVR deletion mutants in vivo. Journal of Virology. 83:384-395. ( Abstract)
  • Cao D, Y.W. Huang, and X.J. Meng (2010). The nucleotides on the stem-loop RNA structure in the junction region of the hepatitis E virus (HEV) genome are critical for virus replication. Journal of Virology. 84(24):13040–13044. (Abstract)
  • Córdoba L, Huang YW, Opriessnig T, Harral KK, Beach NM, Finkielstein CV, Emerson SU, Meng XJ (2011). Three amino acid mutations (F51L, T59A, and S390L) in the capsid protein of the hepatitis E virus collectively contribute to virus attenuation. Journal of Virology. 85(11):5338-49. (Abstract)
  • Pudupakam RS, Kenney SP, Córdoba L, Huang YW, Dryman BA, Leroith T, Pierson FW, Meng XJ (2011). Mutational Analysis of the Hypervariable Region of the Hepatitis E Virus Reveals Its Involvement in the Efficiency of Viral RNA Replication. Journal of Virology. 85(19):10031-40. (Abstract)
  • Kenney SP, Pudupakam RS, Huang YW, Pierson FW, LeRoith T, Meng XJ (2012). The PSAP motif within the ORF3 protein of an avian strain of the hepatitis E virus is not critical for viral infectivity in vivo but plays a role in virus release. Journal of Virology. 86(10):5637-46. (Abstract)
  • Karpe YA, Meng XJ (2012). Hepatitis E virus replication requires an active ubiquitin-proteasome system. Journal of Virology. 86(10):5948-52. (Abstract)
  • Kenney SP, Meng XJ (2015). The lysine residues within the human ribosomal protein S17 sequence naturally inserted into the viral nonstructural protein of a unique strain of hepatitis E virus are important for enhanced virus replication.  Journal of Virology89(7):3793-803. (Abstract)
  • Meng XJ (2016). Expanding Host Range and Cross-Species Infection of Hepatitis E Virus. PLoS Pathogens. 12(8):e1005695. (Abstract)
  • Sooryanarain H, Rogers AJ, Cao D, Haac MER, Karpe YA, Meng XJ (2017). ISG15 Modulates Type I Interferon Signaling and the Antiviral Response during Hepatitis E Virus Replication. Journal of Virology. 2017 Sep 12;91(19). pii: e00621-17. doi: 10.1128/JVI.00621-17. (Abstract)
  • Cao D, Cao QM, Subramaniam S, Yugo DM, Heffron CL, Rogers AJ, Kenney SP, Tian D, Matzinger SR, Overend C, Catanzaro N, LeRoith T, Wang H, Piñeyro P, Lindstrom N, Clark-Deener S, Yuan L, Meng XJ (2018). Pig model mimicking chronic hepatitis E virus infection in immunocompromised patients to assess immune correlates during chronicity. Proceedings of the National Academy of Sciences USA. 114(27):6914-6923. (Abstract)
  • Yugo DM, Heffron CL, Ryu J, Uh K, Subramaniam S, Matzinger SR, Overend C, Cao D, Kenney SP, Sooryanarain H, Cecere T, LeRoith T, Yuan L, Jue N, Clark-Deener S, Lee K, Meng XJ (2018). Infection Dynamics of Hepatitis E Virus in Wild-Type and Immunoglobulin Heavy Chain Knockout JH -/- Gnotobiotic Piglets. Journal of Virology. 2018 Oct 12;92(21). pii: e01208-18. doi: 10.1128/JVI.01208-18. (Abstract)
  • Sooryanarain H, Heffron CL, and X.J. Meng (2020). The U-rich untranslated region of the hepatitis E virus induces differential type I and type III interferon responses in a host cell- dependent manner. mBio. 11:e03103-19. (Abstract)

II: Porcine Circovirus (PCV)

Type-2 porcine circovirus (PCV2) is the primary causative agent of Porcine Circovirus-Associated Diseases (PCVAD) in pigs worldwide. The disease occurs as a low morbidity but high case fatality disease of 5 to 16 week-old pigs. PCVAD poses a serious economic impact on the global swine industry. We have developed the first USDA fully-licensed commercial vaccine, Fostera™ PCV (formerly Suvaxyn® PCV2 One Dose™), to combat PCV2 infection and PCVAD. Our current research focuses on understanding of the molecular basis of PCV2 pathogenesis and replication and development of a second-generation vaccine against PCV2.

Representative publications:

  • Fenaux, M., P.G. Halbur, G. Haqshenas, R. Royer, P. Nawagitgul, M. Gill, T.E. Toth, and X.J. Meng (2002). The cloned genomic DNA of the type-2 porcine circovirus (PCV-2) is infectious when injected into the liver and lymph nodes of SPF pigs: characterization of clinical course, virus distribution and pathological lesions. Journal of Virology. 76:541-551. ( Abstract).
  • Fenaux M, Opriessnig T, Halbur PG, and Meng XJ (2003). Immunogenicity and pathogenicity of chimeric infectious DNA clones of pathogenic porcine circovirus type 2 (PCV2) and nonpathogenic PCV1 in weanling pigs. Journal of Virology. 77:11232-11243. ( Abstract).
  • Lekcharoensuk P, Morozov I, Paul PS, Thangthumniyom N, Wajjawalku W, and Meng XJ (2004). Epitope mapping of the major capsid protein of type 2 porcine circovirus (PCV2) by using chimeric PCV1 and PCV2. Journal of Virology. 78:8135-8145 ( Abstract).
  • Fenaux M, Opriessnig T, Halbur PG, Elvinger F, and Meng XJ (2004). A chimeric porcine circovirus (PCV) with the immunogenic capsid gene of the pathogenic PCV type 2 (PCV2) cloned into the genomic backbone of the nonpathogenic PCV1 induces protective immunity against PCV2 infection in pigs. Journal of Virology. 78:6297-6303. ( Abstract).
  • Fenaux M, Opriessnig T, Halbur PG, Elvinger F, Meng XJ. 2004. Two amino acid mutations in the capsid protein of type 2 porcine circovirus (PCV2) enhanced PCV2 replication in vitro and attenuated the virus in vivo. Journal of Virology. 78:13440-13446. ( Abstract).
  • Gillespie J, N.M. Juhan, J. DiCristina, K.F. Key, S. Ramamoorthy, and X.J. Meng ( 2008). A genetically-engineered chimeric vaccine against porcine circovirus type 2 (PCV2) is genetically stable in vitro and in vivo. Vaccine. 26:4231-4236. ( Abstract)
  • Beach NM, Juhan NM, Cordoba L, and X.J. Meng (2010). Replacement of the replication factors of porcine circovirus (PCV) type 2 with those of PCV type 1 greatly enhances viral replication in vitro. Journal of Virology. 84(17):8986-8989. (Abstract)
  • Beach NM, Ramamoorthy S, Opriessnig T, Wu SQ, and X.J. Meng (2010). Novel chimeric porcine circovirus (PCV) with the capsid gene of the emerging PCV2b subtype cloned in the genomic backbone of the non-pathogenic PCV1 is attenuated in vivo and induces protective and cross-protective immunity against PCV2b and PCV2a subtypes in pigs. Vaccine. 29(2):221-232. (Abstract)
  • Beach NM, Smith SM, Ramamoorthy S, Meng XJ (2011). Chimeric porcine circoviruses (PCV) containing amino acid epitope tags in the C terminus of the capsid gene are infectious and elicit both anti-epitope tag antibodies and anti-PCV type 2 neutralizing antibodies in pigs. Journal of Virology. 85(9):4591-5. ( Abstract)
  • Opriessnig T, Gerber PF, Xiao CT, Halbur PG, Matzinger SR, Meng XJ (2014). Commercial PCV2a-based vaccines are effective in protecting naturally PCV2b-infected finisher pigs against experimental challenge with a 2012 mutant PCV2. Vaccine. 32(34):4342-4348. (Abstract)
  • Matzinger SR, Opriessnig T, Xiao CT, Catanzaro N, Beach NM, Slade DE, Nitzel GP, Meng XJ (2016). A chimeric virus created by DNA shuffling of the capsid genes of different subtypes of porcine circovirus type 2 (PCV2) in the backbone of the non-pathogenic PCV1 induces protective immunity against the predominant PCV2b and the emerging PCV2d in pigs.  Virology. 498:82-93. (Abstract)
  • Opriessnig T, Xiao CT, Halbur PG, Gerber PF, Matzinger SR, Meng XJ (2017). A commercial porcine circovirus (PCV) type 2a-based vaccine reduces PCV2d viremia and shedding and prevents PCV2d transmission to naïve pigs under experimental conditions. Vaccine. 35(2):248-254. (Abstract)
  • Opriessnig T, Castro AMMG, Karuppanan AK, Gauger PC, Halbur PG, Matzinger SR Meng XJ (2019). A Porcine circovirus type 2b (PCV2b)-based experimental vaccine is effective in the PCV2b-Mycoplasma hyopneumoniae coinfection pig model. Vaccine. 37(44):6688-6695. ( Abstract)

III: Porcine Reproductive and Respiratory Syndrome Virus (PRRSV)

Porcine reproductive and respiratory syndrome (PRRS), was first recognized in 1987 in the United States. The disease was characterized by severe reproductive failure in sows and respiratory diseases in young pigs. PRRS has been devastating to the global swine industry causing tremendous economic losses. The causative agent of PRRS, porcine reproductive and respiratory syndrome virus (PRRSV), is a single-stranded positive-sense RNA virus in the family of Arteriviridae. Despite intensive research, PRRS remains difficult to control. Our current research focuses on understanding the molecular mechanisms of PRRSV pathogenesis and replication, and developing effective vaccines against PRRSV.

Representative publications:

  • Cao QM, Tian D, Heffron CL, Subramaniam S, Opriessnig T, Foss DL, Calvert JG, and Meng XJ. 2019. Cytotoxic T lymphocyte epitopes identified from a contemporary strain of porcine reproductive and respiratory syndrome virus enhance CD4+CD8+ T, CD8+ T, and γδ T cell response. Virology. 538:35-44. (Abstract)
  • Cao QM, Ni YY, Cao D, Tian D, Yugo DM, Heffron CL, Overend C, Subramaniam S, Rogers AJ, Catanzaro N, LeRoith T, Roberts PC, Meng XJ (2018). Recombinant Porcine Reproductive and Respiratory Syndrome Virus Expressing Membrane-Bound Interleukin-15 as an Immunomodulatory Adjuvant Enhances NK and γδ T Cell Responses and Confers Heterologous Protection.  Journal of Virology. 2018 Jun 13;92(13). pii: e00007-18. doi: 10.1128/JVI.00007-18. (Abstract)
  • Tian D, Cao D, Lynn Heffron C, Yugo DM, Rogers AJ, Overend C, Matzinger SR, Subramaniam S, Opriessnig T, LeRoith T, Meng XJ (2017). Enhancing heterologous protection in pigs vaccinated with chimeric porcine reproductive and respiratory syndrome virus containing the full-length sequences of shuffled structural genes of multiple heterologous strains.  Vaccine. 35:2427-2434. (Abstract)
  • Cao QM, Subramaniam S, Ni YY, Cao D, Meng XJ (2016). The non-structural protein Nsp2TF of porcine reproductive and respiratory syndromevirus down-regulates the expression of Swine Leukocyte Antigen class I.  Virology. 491:115-24. (Abstract)
  • Tian D, Meng XJ (2016). Amino acid residues Ala283 and His421 in the RNA-dependent RNA polymerase ofporcine reproductive and respiratory syndrome virus play important roles in viral ribavirin sensitivity and quasispecies diversity. Journal of General Virology. 97(1):53-59. (Abstract)
  • Tian D, Ni YY, Zhou L, Opriessnig T, Cao D, Piñeyro P, Yugo DM, Overend C, Cao Q, Lynn Heffron C, Halbur PG, Pearce DS, Calvert JG, Meng XJ (2015). Chimeric porcine reproductive and respiratory syndrome virus containing shuffled multiple envelope genes confers cross-protection in pigs. Virology. 485:402-413. (Abstract)
  • Ni YY, Z. Zhao, T. Opriessnig, S. Subramaniam, L. Zhou, D. Cao, Q. Cao, H. Yang, and Meng XJ. 2014. Computer-aided codon-pairs deoptimization of the major envelope GP5 gene attenuates porcine reproductive and respiratory syndrome virus. Virology. 450-451:132-139. (Abstract)
  • Ni YY, Opriessnig T, Zhou L, Cao D, Huang YW, Halbur PG, Meng XJ (2013). Attenuation of porcine reproductive and respiratory syndrome virus by molecular breeding of the virus envelope genes from genetically divergent strains. Journal of Virology. 87(1): 304-313. ( Abstract)
  • Zhou L, Ni YY, Piñeyro P, Sanford BJ, Cossaboom CM, Dryman BA, Huang YW, Cao DJ, Meng XJ (2012). DNA shuffling of the GP3 genes of porcine reproductive and respiratory syndrome virus (PRRSV) produces a chimeric virus with an improved cross-neutralizing ability against a heterologous PRRSV strain. Virology. 434(1):96-109. ( Abstract)
  • Ni YY, Huang YW, Cao D, Opriessnig T, Meng XJ (2011). Establishment of a DNA-launched infectious clone for a highly pneumovirulent strain of type 2 porcine reproductive and respiratory syndrome virus: identification and in vitro and in vivo characterization of a large spontaneous deletion in the nsp2 region. Virus Research. 160(1-2):264-73. (Abstract)
  • Huang YW, and X.J. Meng (2010). Novel strategies and approaches to develop the next generation of vaccines against porcine reproductive and respiratory syndrome virus (PRRSV). Virus Research. 154(1-2):141-149. ( Abstract)
  • Huang YW, Fang Y, Meng XJ (2009). Identification and characterization of a porcine monocytic cell line supporting porcine reproductive and respiratory syndrome virus (PRRSV) replication and progeny virion production by using an improved DNA-launched PRRSV reverse genetics system. Virus Research. 145(1):1-8. ( Abstract)
  • Opriessnig T., P.G. Halbur, K.J. Yoon, R. Pogranichniy, E. Vaughn, K. Harmon, R.Evans, K.F. Key, F. Pallares, P. Thomas, and X.J. Meng (2002). Comparison of molecular and biological characteristics of a modified live PRRSV vaccine (RespPRRS/Repro™), the parent strain of the vaccine (ATCC VR2332), ATCC VR2385, and two recent field isolates of PRRSV. Journal of Virology. 76:11837-11844. (Abstract)
  • Meng X.J., P.S. Paul, I. Morozov, and P.G. Halbur (1996). A nested set of six or seven subgenomic mRNAs is formed in cells infected with different isolates of porcine reproductive and respiratory syndrome virus. Journal of General Virology. 77:1265-1270. ( Abstract)
  • Meng X.J., P.S. Paul, P.G. Halbur, and I. Morozov (1995). Sequence comparison of open reading frames 2 to 5 of low and high virulence U.S. isolates of porcine reproductive and respiratory syndrome viruses. Journal of General Virology. 76:3181-3188. ( Abstract)
  • Meng X.J., P.S. Paul, and P.G. Halbur (1994). Molecular cloning and nucleotide sequencing of the 3' terminal genomic RNA of porcine reproductive and respiratory syndrome virus. Journal of General Virology. 75:1795-1801. (Abstract).

IV: Torque Teno Sus Virus (TTSuV)

Torque teno sus virus (TTSuV) belongs to the family Anelloviridae. The pathogenic potential of TTSuV remains unknown. Our current research focuses on understanding the mechanisms of replication and biology of TTSuVs.

Representative publications:

  • Rogers AJ, Huang YW, Heffron CL, Opriessnig T, Patterson AR, Meng XJ (2016). Prevalence of the Novel Torque Teno Sus Virus Species k2b from Pigs in the United States and Lack of Association with Post-Weaning Multisystemic Wasting Syndrome or Mulberry Heart Disease.  Transboundary and Emerging Diseases. 2016 Nov 23. doi: 10.1111/tbed.12586. (Abstract)
  • Huang YW, Patterson AR, Opriessnig T, Dryman BA, Gallei A, Harrall KK, Vaughn EM, Roof MB, Meng XJ (2012). Rescue of a porcine anellovirus (torque teno sus virus 2) from cloned genomic DNA in pigs. Journal of Virology. 86(11):6042-54. (Abstract)
  • Huang YW, Harrall KK, Dryman BA, Opriessnig T, Vaughn EM, Roof MB, Meng XJ (2012). Serological profile of torque teno sus virus species 1 (TTSuV1) in pigs and antigenic relationships between two TTSuV1 genotypes (1a and 1b), between two species (TTSuV1 and -2), and between porcine and human anelloviruses. Journal of Virology. 86(19):10628-39. (Abstract)
  • Huang YW, Harrall KK, Dryman BA, Beach NM, Kenney SP, Opriessnig T, Vaughn EM, Roof MB, Meng XJ (2011). Expression of the putative ORF1 capsid protein of Torque teno sus virus 2 (TTSuV2) and development of Western blot and ELISA serodiagnostic assays: correlation between TTSuV2 viral load and IgG antibody level in pigs. Virus Research. 158(1-2):79-88. ( Abstract)
  • Huang YW, Ni YY, Dryman BA, Meng XJ (2010). Multiple infection of porcine Torque teno virus in a single pig and characterization of the full-length genomic sequences of four U.S. prototype PTTV strains: Implication for genotyping of PTTV. Virology. 396(2):289-297. ( Abstract)
  • McKeown NE, Fenaux M, Halbur PG, and Meng XJ (2004). Molecular characterization of porcine TT virus, an orphan virus, in pigs from six different countries. Veterinary Microbiology. 104:113-117. ( Abstract)

V: Porcine Epidemic Diarrhea Virus (PEDV)

The sudden emergence of porcine epidemic diarrhea virus (PEDV), a coronavirus, for the first time in the United States in 2013 causes significant economic concerns. Our current research focuses on understanding the mechanism of pathogenesis, and developing effective vaccines against PEDV.

Representative publications:

  • Subramaniam S, Yugo DM, Heffron CL, Rogers AJ, Sooryanarain H, LeRoith T, Overend C, Cao D, Meng XJ (2018). Vaccination of sows with a dendritic cell-targeted porcine epidemic diarrhea virus S1 protein-based candidate vaccine reduced viral shedding but exacerbated gross pathological lesions in suckling neonatal piglets.  Journal of General Virology. 99(2):230-239. (Abstract)
  • Subramaniam S, Cao D, Tian D, Cao QM, Overend C, Yugo DM, Matzinger SR, Rogers AJ, Heffron CL, Catanzaro N, Kenney SP, Opriessnig T, Huang YW, Labarque G, Wu SQ, Meng XJ (2017). Efficient priming of CD4 T cells by Langerin-expressing dendritic cells targeted withporcine epidemic diarrhea virus spike protein domains in pigs. Virus Research. 227:212-219. (Abstract)
  • Gerber PF, Xiao CT, Lager K, Crawford K, Kulshreshtha V, Cao D, Meng XJ, Opriessnig T (2016). Increased frequency of porcine epidemic diarrhea virus shedding and lesions in suckling pigs compared to nursery pigs and protective immunity in nursery pigs after homologous re-challenge. Veterinary  Research. 47(1):118. (Abstract)
  • Huang YW, A.W. Dickerman, P. Pineyro, L. Li, L. Fang, R. Kiehne, T. Opriessnig, and X.J. Meng. 2013. Origin, evolution, and genotyping of emergent porcine endemic diarrhea virus (PEDV) strains in the United States. mBio. 4(5):e00737-13. doi:10.1128/mBio.00737-13. (Abstract)