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Equipment used in the CREATE Lab

Faculty members seeking information about the research services available through the CREATE laboratory should see the CREATE lab profile on the R&GS web site.

Laboratory Instrumentation

The CREATE Laboratory equipment includes:

  • Kryo-10 Cryopreservation System
    Semen, oocyte, and embryo cryopreservation; design and flexibility applicable to all species. (view image)
  • Nikon TE-300 Inverted Phase Contrast with Hoffman Modulation of Optics and Illumination Micromanipulation System
    For oocyte and embryo manipulations, IVF, ICSI. (view image)
  • Large Capacity Liquid Nitrogen Storage Tank
    Storage of oocytes, embryos, and semen. (view image)
  • Basic Laboratory Support Equipment:
    Refrigerated benchtop centrifuge, -70oC freezer, explosion-proof refrigerator, environmental chamber/incubator, C02 water-jacketed incubator, purified air filtration clean bench, sterile hood.
  • Phantom for Stallion Semen Collection
    Housed at VTH, VMRCVM, Blacksburg.
  • Sperm Concentration Analysis
    Animal Reproduction Systems Densimeter and HRI Sperm Counter (view image)
  • Storz Laparoscope
    Rigid 33cm, 10mm dia. With 175W xenon light source and videorecording attachment; for small animal and small domestic ruminant oocyte recoveries, embryo transfer, and intrauterine inseminations.
  • Macintosh 8600/300 computer with IBM-PC compatibility card, 2.4 GB hard drive, and Apple 16-inch color monitor
    For semen inventory system maintenance, research data storage and analysis.
  • HTM Ivos Photomotility Analyzer
    Computer Assisted Motility Analyzer
  • Epics V Flow Cytometer
    Flow Cytometry Resource Laboratory at VMRCVM; used for sperm function and fertility analyses.
  • Olympus BX-51 Compound Microscope
    Brightfield, phase contrast, Differential Interference Contrast optics, ultraviolet illumination and Spot RT digital camera.

Instrumentation Images

Micromanipulation System Micromanipulation System ****

Automated Cryopreservation (LN2) System Automated Cryopreservation (LN2) System ****

Liquid Nitrogen (LN2) Storage System Liquid Nitrogen (LN2) Storage System ****

Sperm Concentration Analysis Sperm Concentration Analysis ****