F. William Pierson

Principal Investigator/Program Director (Last, first, middle): Pierson, F. William
BIOGRAPHICAL SKETCH Provide the following information for the key personnel in the order listed for Form Page 2. Follow the sample format for each person. DO NOT EXCEED FOUR PAGES.

NAME F. William Pierson		POSITION TITLE Associate Professor
EDUCATION/TRAINING (Begin with baccalaureate or other initial professional education, such as nursing, and include postdoctoral training.)
INSTITUTION AND LOCATION	DEGREE (if applicable)		YEAR(s)	FIELD OF STUDY
University of Delaware	BS		75-78	Animal Science
Purdue University	MS		78-80	Avian Physiology
Virginia Tech	DVM		80-84	Clinical Medicine
Virginia Tech	PhD		87-93	Microbiology/Immunology
American College of Poultry Veterinarians	Diplomate		94	Poultry Medicine

NOTE: The Biographical Sketch may not exceed four pages. Items A and B (together) may not exceed two of the four-page limit. Follow the formats and instructions on the attached sample.

A. Positions and Honors. List in chronological order previous positions, concluding with your present position. List any honors. Include present membership on any Federal Government public advisory committee.

Positions

1984-1987: Associate Veterinarian,Londonderry Animal Hospital, Middletown, PA.

1987-1990: Graduate Research Assistant Department of Large Animal Clinical Sciences, Virginia‑Maryland Regional College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, VA.

1990-1991: Research Associate, non-tenure track, Department of Large Animal Clinical Sciences, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, VA.

1991-1993: Research Scientist, non-tenure track, Department of Large Animal Clinical Sciences, Virginia‑Maryland Regional College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, VA.

1993-1998: Assistant Professor of Avian Medicine, tenure track, Department of Large Animal Clinical Sciences, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, VA.

1998-Present: Associate Professor of Avian Medicine, tenured, Department of Large Animal Clinical Sciences, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, VA.

1999-Present: Adjunct Associate Professor, Maryland Campus, Virginia-Maryland Regional College of Veterinary Medicine, University of Maryland, College Park, MD.

Honors

1979: Certificate of Excellence for Graduate Research Presentation, Poultry Science Association, 1979.

1980: Gamma Sigma Delta, Honor Society of Agriculture, 1980.

1980: Phi Kappa Phi, National Honor Society, 1980.

1984: Phi Zeta, Honor Society of Veterinary Medicine, 1984.

1988: First Place, Graduate Research Award Competition, Virginia-Maryland Regional College of Veterinary Medicine, 1988.

1991: Sigma Xi, Scientific Research Society, 1991.

1992: College Teaching Award, Virginia-Maryland Regional College of Veterinary Medicine, 1992.

1992: Certificate of Teaching Excellence, Virginia Polytechnic Institute and State University, 1992.

B. Selected peer-reviewed publications (in chronological order). Do not include publications submitted or in preparation.

(Last 5 years)

1. Hegngi, F.N., J. Doerr, T.S. Cummings, R.D. Schwartz, G. Saunders, A. Zajac, C.T. Larsen, and F.W. Pierson, 1999. The effectiveness of benzimidazole derivatives for the treatment and prevention of histomonosis (blackhead) in turkeys. Vet Parasit 81:29-37.

2. Lindsay, D.S., C.T. Larsen, A.M. Zajac, and F.W. Pierson, 1999. Experimental Cochlosoma anatis infections in poultry. Vet Parasit 81:21-27.

3. Boa-Amponsem, E.A. Dunnington, F.W. Pierson, C.T. Larsen, and P.B. Siegel, 2000. Antibody responses to different dosages of sheep red blood cells in lines of chickens selected for high and low antibody response to sheep red blood cells. Poultry Sci 79:159-162.

4. Tian, Y. F.W. Pierson, S.M. Boyle, and C.L. Cramer, 2000. Transgenic plants as edible vaccines. Proceedings of the XXI World’s Poultry Congress, Montreal, Canada, CD-ROM, Biotechnology and Vaccine Development Session, pp 27-47.

5. Hrubec, T.C., J.M. Whichard, C.T. Larsen, and F.W. Pierson, 2002. Plasma vs serum: specific differences in biochemical analyte values. J Avian Med and Surg 16:101-105.

6. Altekruse, S.F., F. Elvinger, J. Eifert, D. Karunakaran, F. W. Pierson, and N. Sriranganathan, 2002. Antimicrobial susceptibilities of Escherichia coli strains from a turkey operation. J Am Vet Med Assoc 221:411-416.

7. Altekruse, S.F., F. Elvinger, C. Debroy, F.W. Pierson, J.D. Eifert, D. Karunakaran, N.Sriranganathan, 2002. Pathogenic and fecal Escherichia coli strains from turkeys in a commercial operation. Avian Dis 46:562-569.

8. Haqshenas, G., F.F. Huang, M. Fenaux, D. Guenette, F.W. Pierson, C. Larsen, H.L. Shivaprasad, T.E. Toth, and X.J. Meng, 2002. The putative capsid protein of the newly identified avian hepatitis E virus shares antigenic epitopes with that of swine and human hepatitis E viruses and big liver and spleen disease virus. J Gen Virology 83:2201-2209.

9. Huang, F.F., G. Haqshenas, D.K. Guenette, S.K. Schommer, F.W. Pierson, T.E. Toth, and X.J. Meng, 2002. Detection by RT-PCR and genetic characterization of field isolates of swine hepatitis E virus from pigs in different geographic regions of the United States. J Clin Microbiol 40:1326-1332.

10. Huang, F.F., G. Haqshenas, H.L. Shivaprasad, D.K. Guenette, P.R. Woolkock, C.T. Larsen, F.W. Pierson, F. Elvinger, T.E. Toth, and X.J. Meng, 2002. Heterogeneity and seroprevalence of the newly identified avian hepatitis E virus from chickens in the United States. J Clin Microbiol 40:4197-4202.

11. Gross, W.B., P.B. Siegel, and F.W. Pierson, 2002. Effects of genetic selection for high and low antibody response on resistance to a variety of disease challenges and the relationship of resource allocation. Avian Dis 46:1007-1010.

12. Whichard, J.M., N. Sriranganathan, and F.W. Pierson, 2002. Bacteriophage Felix O1: Suppression of Salmonella growth by wild-type and large-type plaque isolates in liquid culture and on poultry frankfurters. J. Food Protection 66:220-225.

13. Sun, Z.F., F.F. Huang, P.G. Halbur, S.K. Schommer, F.W. Pierson, T.E. Toth, and X.J. Meng, 2003. Use of heteroduplex mobility assays (HMA) for pre-sequencing screening and identification of variant strains of avian and swine hepatitis E viruses. Vet Micrbiology 96:165-176.

14. Eifert, J.D., R.M. Castle, F.W. Pierson, C.T. Larsen, C.R. Hackney, 2003. Comparison of sampling techniques for detection of Arcobacter butzleri from chickens. Poultry Sci 82:1898-1902.

15. Sun, Z.F., C.T. Larsen, A. Dunlop, F. Huang, F.W. Pierson, T.E. Toth, and X.J. meng, 2004. Genetic identification of avian hepatitis E virus (avian HEV) from healthy chicken flocks and characterization f the capsid gene of 14 avian HEV isolates from chicken with hepatitis-splenomegaly syndrome in different grographic regions of the United States. J Gen Virology 85:693-700.

16. Sun Z.F., C.T. Larsen, F.F. Huang, P. Billam, F.W. Pierson, T.E. Toth, and X.J. Meng, 2004. Generation and infectivity titration of an infectious stock of avian hepatitis E virus (avian HEV) in chickens and cross-species infection of turkeys with avian HEV. J Clin Microbiology 42:2351-2352.

17. Huang, F.F., A. Sun, S.U. Emerson, R.H. Purcell, H.L. Shivaprasad, F.W. Pierson, T.E. Toth, and X.J. Meng, 2004. Determination and analyses ogf the complete genomic sequence of avian hepatitis E virus and attempts to experimentally infect rhesus monkeys with avian HEV. J Gen Virology 85:1609-1618.

18. Smith, E.J., E.C. Long, P. Billam, F.W. Pierson, D.P. Sponenberg, C.T. Larsen, and R. Gogal, 2004. Molecular analysis of relatedness among five turkey varieties. Biochemical Genetics 43:35-48.

19. Falkingham, J.O., W.B. Gross, and F.W. Pierson, 2004. Effect of different cell fractions of Mycobacterium avium and vaccination regimens on Mycobacterium avian infection. Scandinavian Journal of Immunology (in press).

20. McQuiston, J.H., L.P. Garber, B.A. Porter-Spalding, J.W. Hahn, F.W. Pierson, S.H. Wainwright, D.A. Senne, T.J. Brignole, B.L. Akey, T.J. Holt, 2004. Risk factors for spread of low pathogenicity H7N2 avian influenza virus among commercial poultry farms in Virginia, 2002. J Amer Vet Med Asso (in press).

C. Research Support. List selected ongoing or completed (during the last three years) research projects (federal and non-federal support). Begin with the projects that are most relevant to the research proposed in this application. Briefly indicate the overall goals of the projects and your role (e.g. PI, Co-Investigator, Consultant) in the research project. Do not list award amounts or percent effort in projects.

Sponenberg, D.P., and F.W. Pierson (Co-PI), 200-2002. Documenting the immunological health of standard turkey varieties versus industrial turkey stocks, American Livestock Breeds Conservancy, Purpose: to compare basic immunological parameters in phenotypically divergent lines of turkeys.

R.M. Gogal, F.W. Pierson (Co-PI) and C.T. Larsen, 2002-2004. Comparison of immunological profiles between commercial and standard/historic lines of turkeys, American Livestock Breeds Conservancy. Purpose: to perform in depth immunological evaluations and genotypic analyses to differentiate between heritage (primitive) and commercial varieties of turkeys and to document and preserve immunological competence in the gene pool.

Meng, X.J., F.W.Pierson (Co-PI), and T.E. Toth, 2002-2004.. Serological and molecular epidemiology of avian hepatitis E virus, an emerging virus causing hepatitis-splenomegaly syndrome in chickens. USDA / NRI. Purpose: to assess the molecular and serological prevalence of avian hepatitis E virus in chickens.

Meng, X.J., F.W. Pierson (Co-I), T.E. Toth, and H.L. Shivaprasad, 2002-2006. A chicken model for hepatitis E virus pathogenesis. NIAID/NIH. . Purpose: to assess the zoonotic risk of avian hepatitis E virus and evaluate chickens as a model for the study of human hepatitis E virus replication and pathogenesis.

Pierson, F.W., (PI) 2002-2004. Field service and applied research grant - primary project: latency of hemorrhagic enteritis virus. Cargill Turkey Products, Inc. Purpose: to determine the potential for latency and recrudescence of hemorrhagic enteritis virus of turkeys (Siadenovirus) and perform molecular / genomic comparisons between virulent and avirulent strains.

Pierson, F.W., C.T. Larsen, and T. Beacorn, (PI) 2002-2004. Field service and applied research grant - primary project: the effects of stress on response to hemorrhagic enteritis vaccine. Pilgrims Pride, Inc. Purpose: to determine the effects of social stress on immune function and response to hemorrhagic enteritis virus (a lymphotrophic, lymphocytopathic virus) infection.

Pierson, F.W., (PI) 2002-2003 Field service and applied research grant - primary project: the combined effects of stress and vitamin C on response to hemorrhagic enterits vaccine. Carroll's Foods of Virginia. Purpose: to determine the effects of stress and vitamin C supplementation on immune function and response to vaccination with avirulent (live) hemorrhagic enteritis virus.

Ruszler, P, F.W. Pierson (Co-PI), C. Novak, 2003-2004. Molting Hens Using a Non-fasting Program Compared to a 4-Day Fasting Program and Incidence of Salmonella Enteriditis in Post-Molt Hens U.S. Poultry and Egg Association. Purpose: to evaluate the effect of 4 different molting diets on Salmonella enteriditis colonization, shedding, and lateral transfer in laying hens.

Hovingh, E., and F.W. Pierson. (Co-PI) 2003-2004. Evaluation of an Alcohol-based, Waterless Hand Sanitizer in Petting Fair/Zoo and Large Animal Clinical Veterinary Hospital Environments, GOJO Industries. Purpose: to evaluate effectiveness of waterless hand sanitizer against bacteria found on the hands of people in contact with animals in agricultural and veterinary settings.

Elvinger, F., and F.W. Pierson (Co-PI), 2003-2005. Epidemiological and Economic Assessment Study of the Performance of the Screening and Diagnostic Tests Used During Virginia's Low Pathogenic Avian Influenza Outbreak to Identify Infected Flocks, USDA . Purpose: to evaluate the efficiency of diagnostic sampling and testing (antigen capture, AGID, RT-PCR, isolation) in a recent outbreak of avian influenza.