Publications from the Aquatic Medicine Program


Mycobacteriosis

Pasnik D.J. and S.A. Smith. 2005. Immunogenic and protective effects of a DNA vaccine for Mycobacterium marinum. Veterinary Immunology and Immunopathology 103:195-206.

Pasnik, D.J., R. Vemulapalli, S.A. Smith and G.G. Schurig. 2003. A recombinant vaccine expressing a mammalian Mycobacterium sp. antigen is immunostimulatory but not protective in striped bass. Veterinary Immunology and Immunopathology 95:43-52.

Harms, C.A., K.E. Howard, J.C. Wolf, S.A. Smith and S. Kennedy-Stoskopf. 2003. Transforming growth factor-B response to mycobacterial infection in striped bass Morone saxatilis and hybrid tilapia Oreochromis spp. Veterinary Immunology and Immunopathology 95:155-163.

Hughes, K.P, R.B. Duncan and S.A. Smith. 2002. Renomegaly associated with a mycobacterial infection in summer flounder, Paralichthys dentatus. Fish Pathology 37:83-86.

Hughes, K.P., R.B. Duncan and S.A. Smith. 2002. Mass in oral cavity of cultured summer flounder, Paralichthys dentatus. Lab Animal 31:25-27.

Wolf, J.C. and S.A. Smith. 1999. Comparative severity of experimentally-induced mycobacteriosis in striped bass Morone saxatilis and hybrid tilapia Oreochromis spp. Diseases of Aquatic Organisms 38:191-200.

Smith, S.A. 1997. Mycobacterial infections in pet fish. Seminars in Avian and Exotic Pet Medicine 6:40-45.

Immunology

Hrubec, T.C., D. Ward, S.A. Smith and J.L. Robertson. 2004. Age-related changes in humoral immune response of hybrid striped bass (Morone chrysops x Morone saxatilis). Veterinary Immunology and Immunopathology 101:103-108.

Smith D.A., G. Schurig, S.A. Smith and S.D. Holladay. 1999. The hemolytic plaque-forming cell assay in tilapia (Oreochromis niloticus) exposed to benzo(a)pyrene: Enhanced or depressed plaque formation depends on dosing schedule. Toxicology Methods 9:57-70.

Gogal, R.M., B.J. Smith, J.L. Robertson, S.A. Smith and S.D. Holladay. 1999. Tilapia (Oreochromis niloticus) dosed with azathioprine display immune effects similar to those seen in mammals, including apoptosis. Veterinary Immunology and Immunopathology 68: 211-229.

Smith, S.A., M.G. Levy and E.J. Noga. 1994. Detection of anti-Amyloodinium ocellatum antibody from cultured hybrid striped bass during an epizootic of amyloodiniosis. Journal of Aquatic Animal Health 6:79-81.

Hematology and Blood Chemistry

Hrubec, T.C. and S.A. Smith. 2004. Hematology and blood chemistry reference intervals for yellow perch (Perca flavescens) raised in recirculation systems. International Journal of Recirculating Aquaculture 5:29-42.

Dye, V.A, T.C. Hrubec, J.L. Dunn and S.A. Smith. 2001. Hematology and serum chemistry values for winter flounder (Pleuronectes americanus). International Journal of Recirculation Aquaculture 2:37-50.

Hrubec, T.C., S.A. Smith and J.L. Robertson. 2001. Age-related changes in hematology and plasma chemistry values of hybrid striped bass (Morone chrysops x Morone saxatilis). Veterinary Clinical Pathology 30:8-15.

Hrubec, T.C. and S.A.Smith. 2000. Hematology and plasma chemistry values for production tilapia (Oreochromis hybrid) raised in a recirculation system. International Journal of Recirculation Aquaculture 1:5-14.

Hrubec, T.C., J. Cardinale and S.A. Smith. 2000. Hematology and plasma chemistry reference intervals for cultured tilapia (Oreochromis hybrid). Veterinary Clinical Hematology 29:7-12.

Hrubec, T.C. and S.A. Smith. 1999. Differences between plasma and serum samples for the evaluation of blood chemistry values in rainbow trout, channel catfish, hybrid tilapias and striped bass. Journal of Aquatic Animal Health 11:116-122.

Hrubec, T.C., J.L. Robertson and S.A. Smith. 1997. Effects of temperature on hematologic and biochemical profiles of sunshine bass (Morone chrysops X Morone saxatilis). American Journal of Veterinary Research 58:126-130.

Hrubec, T.C. , J.L. Robertson and S.A. Smith. 1997. Effects of ammonia and nitrate concentration on hematologic and serum biochemical profiles of hybrid striped bass (Morone chrysops X Morone saxatilis). American Journal of Veterinary Research 58:131-134.

Hrubec, T.C., S.A.Smith, J.L. Robertson, B. Feldman, H.P. Veit, G. Libey and M.K. Tinker. 1996. Comparison of hematological reference intervals between culture system and type of hybrid striped bass. American Journal of Veterinary Research 57:618-623.

Hrubec, T.C., S.A. Smith, J.L. Robertson, B. Feldman, H.P. Veit, G. Libey and M.K. Tinker. 1996. Blood biochemical reference intervals for sunshine bass (Morone chrysops X Morone saxatilis) in three culture systems. American Journal of Veterinary Research 57:624-627.

Hrubec, T.C., J.L. Robertson, S.A. Smith and M.K. Tinker. 1996. The effect of temperature and water quality on antibody response to Aeromonas salmonicida in sunshine bass (Morone chrysops X Morone saxatilis). Veterinary Immunology and Immunopathology. 50:157-166.

Toxicology

Smith D.A., G.G. Schurig, S.A. Smith and S.D. Holladay. 1999. Tilapia (Oreochromis niloticus) and rodents exhibit similar patterns of inhibited antibody production following exposure to chemical inhibitors of humoral immune function. Veterinary and Human Toxicology 41:368-373.

Smith D.A., G. Schurig, S.A. Smith and S.D. Holladay. 1999. Inhibited cytotoxic leukocyte acitivity in tilapia (Oreochromis niloticus) following exposure to immunotoxic chemicals. International Journal of Toxicology 8:167-172.

Hart L.J., R.M. Gogal, S.A. Smith, B.J. Smith, J.L. Robertson and S.D. Holladay. 1999. Antiproliferative effects and enhanced apoptosis may contribute to leukocyte hypocellularity in the spleen and pronephros of tilapia (Oreochromis niloticus) exposed to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Toxic Substance Mechanisms 18:21-38.

Holladay, S.D., S.A. Smith, E.G. Besteman, A.S.M.I. Deyab, R.M. Gogal, T. Hrubec, J.L. Robertson and S.A. Ahmed. 1998. Morphological lesions and acute immunotoxic effects in tilapia (Oreochromis nilotica) exposed to benzo(a)pyrene. Veterinary Immunology and Immunopathology 64:69-82.

Hart, L.J., S.A. Smith, B.J. Smith, J.L. Robertson, E.G. Besteman and S.D. Holladay. 1998. Subacute immunotoxic effects of the polycyclic aromatic hydrocarbon 7,12-dimethylbenzanthracene (DMBA) on spleen and pronephros leukocytic cell counts and phagocytic activity in tilapia (Oreochromis niloticus). Aquatic Toxicology 41:17-29.

Hart, L.J., S.A. Smith, B.J. Smith, J.L. Robertson and S.D. Holladay. 1997. Exposure of tilapian fish to the pesticide lindane results in hypocellularity of the primary hematopoietic organ (pronephros) and the spleen without altering activity of phagocytic cells in these organs. Toxicology 118:211-221.

Holladay, S.D., S.A. Smith, H. El-Habback and T. Caceci. 1996. Immune alterations in tilapia (Oreochromis niloticus) following low-level exposure to the organophosphate insecticide, chlorpyrifos. Journal of Aquatic Animal Health 8:104-110.

Augusto, J., B. Smith, S.A. Smith, J. Robertson and R. Reimschuessel. 1996. Gentamicin-induced nephrotoxicity and nephroneogenesis in Oreochromis nilotica, a tilapian fish. Diseases of Aquatic Organisms. 26:49-58.

Pathology and Histology

Pasnik, D.J., S.A. Smith and D.S. Lindsay. 2005. Intestinal coccidiosis in bluegill (Lepomis machrochirus). Journal of Parasitology 91:967-970.

Smith, S.A., K.P. Hughes, and J. Luoma. 2002. Amoebic gill infestation in pallid sturgeon, Scaphirhynchus albus. Bulletin of the European Association of Fish Pathologist 22:400-402.

Smith, B.J., S.A. Smith and C.J. Pfeiffer. 2000. Effects of a recirculating aquaculture system on the gill morphology of hybrid striped bass (Morone saxatilis x M. chrysops). International Journal of Recirculation Aquaculture 1:35-59.

Amyloodiniosis

Smith, S.A., Levy, M.G. and Noga, E.J. 1994. Detection of anti-Amyloodinium ocellatum antibody from cultured hybrid striped bass during an epizootic of amyloodiniosis. Journal of Aquatic Animal Health 6:79-81.

Smith, S.A., E.J. Noga, M.G. Levy and T.M. Gerig. 1993. Effect of serum from the fish, Oreochromis aureus, immunized with dinospores of the parasitic dinoflagellate, Amyloodinium ocellatum, on the motility, infectivity and growth of the parasite in cell culture. Diseases of Aquatic Organisms 15:73-80.

Smith, S.A., D.H. Gebhard, J.M. Housman, M.G. Levy and E.J. Noga. 1993. Isolation, purification and molecular weight determination of serum immunoglobulin from Oreochromis aureus. Journal of Aquatic Animal Health 5:25-35.

Smith, S.A., M.G. Levy and E.J. Noga. 1992. Utilization of affinity-purified antibody in the development of an enzyme-linked immunosorbent assay (ELISA) for the detection of antibody to the parasitic dinoflagellate Amyloodinium ocellatum. Veterinary Parasitology 42:145-155.

Noga, E.J., S.A. Smith and J.H. Landsberg. 1991. Amyloodiniosis in cultured hybrid striped bass (Morone saxatilis x M. chrysops) in North Carolina. Journal of Aquatic Animal Health 3:294-297.

Pfiesteria

Steidinger, K.A., J.M. Burkholder, H.B. Glasgow, C.W. Hobbs, J.K. Garrett, E.W. Truby, E.J. Noga and S.A. Smith. 1996. Pfiesteria piscicida, Gen. et Sp. Nov. (Pfiesteriaceae Fam. Nov.), a new toxic dinoflagellate with a complex life cycle and behavior. Journal of Phycology 32:157-164.

Noga, E.J., S.A. Smith, J.M. Burkholder, C. Hobbs and R.A. Bullis. 1993. A new ichthyotoxic dinoflagellate: cause of acute mortality in aquarium fishes. The Veterinary Record 133:96-97.

Burkholder, J.M., E.J. Noga, C.H. Hobbs, H.B. Glasgow and S.A. Smith. 1992. New "phantom" dinoflagellate is the causative agent of major estuarine fish kills. Nature (London) 358:407-410. (Correction printed in Nature, London, 360:768).

Horseshoe Crabs (Limulus polyphemus)

Smith, S.A. and J. Berkson. 2005. Laboratory Culture and Maintenance of the Horseshoe crab (Limulus polyphemus). Lab Animal 34:27-34.

Hurton, L., J. Berkson and S.A. Smith. 2005. Determination of total hemolymph volume in the horseshoe crab Limulus polyphemus. Marine and Freshwater Behavior and Physiology 38:139-147.

Walls, E.A., J. Berkson and S.A. Smith. 2002. The Horseshoe Crab, Limulus polyphemus: 200 Million Years of Existence, 100 Years of Study. Reviews in Fisheries Science 10:39-73.

Miscellaneous Publications

Hughes, K.P. and S.A. Smith. 2003. Common and emerging diseases in commercially cultured summer flounder, Paralichthys dentatus. Journal of Applied Aquaculture 14:163-178.

Smith, S.A. 2002. Non-lethal clinical techniques used in the diagnosis of fish diseases. Journal of the American Veterinary Medicine Association 220:1203-1206.

Sangmaneedt, S. and S.A. Smith. 2000. In vitro studies on optimal requirements for the growth of Spironucleus vortens, an intestinal parasite of the freshwater angelfish. Diseases of Aquatic Organisms 39:135-141.

Caceci, T., S.A. Smith and T.E. Toth. 1999. Identification of individual prawns with implanted microchip transponders. Aquaculture 180:41-51.

Sangmaneedt, S. and S.A. Smith. 1999. Efficacy of various chemotherapeutic agents on the growth of Spironucleus vortens, an intestinal parasite of the freshwater angelfish. Diseases of Aquatic Organisms 38:39-46.

Noga, E.J., J. Smith and S.A. Smith. 1999. Turbellarian infection of carangids. Journal of Fish Diseases 22:489-491.

Wolf, J.C. and S.A. Smith. 1999. Systemic zygomycosis in farmed tilapia fish. Journal of Comparative Pathology 121:301-306.

Smith, S.A. and B.J. Smith. 1994. Xeroradiographic and radiographic anatomy of the channel catfish, Ictalurus punctatus. Veterinary Radiology and Ultrasound. 35:384-389.

Smith, S.A., T. Caceci and J. Robertson. 1995. Occurrence of rodlet cells and associated lesions in the vascular system of the freshwater angelfish. Journal of Aquatic Animal Health 7:63-69.

Smith, S.A., T. Cacecci, H.E. Marei and H.A. El-Habback. 1995. Observations on rodlet cells found in the vascular system and extra-vascular space of the angelfish (Pterophyllum scalare scalare). Journal of Fish Biology 46:241-254.