Representative publications

Kocher J, Bui T, Giri-Rachman E, Wen K, Li G, Yang Y, Liu F, Tan M, Xia M, Zhong W, Jiang X, Yuan L. 2014. Intranasal P particle vaccine provided partial cross-variant protection against human GII.4 norovirus diarrhea in gnotobiotic pigs. J Virol.2014 Jun 11. pii: JVI.01249-14. [Epub ahead of print]

Wen X, Wen K, Cao D, Li G, Jones RW, Li J, Szu S, Hoshino Y, Yuan L. 2014. Inclusion of a universal tetanus toxoid CD4+ T cell epitope P2 significantly enhanced the immunogenicity of recombinant rotavirus ΔVP8* subunit parenteral vaccines. Vaccine (in press).

Wen K, Tin C, Wang H, Li G, Giri-Rachman E, Yang X, Kocher J, Bui T, Clark-Deener S, Yuan L. 2014. Probiotic Lactobacillus rhamnosusGG enhanced Th1 cellular immunity but did not affect antibody responses in a human gut microbiota transplanted neonatal gnotobiotic pig model. PLoS ONE 9(4): e94504 (doi:10.1371/journal.pone.0094504).

Yang X, Li G, Wen K, Bui T, Liu F, Kocher J, Jortner BS, Vonck M, Pelzer K, Deng J, Zhu R, Li Y, Qian Y, Yuan L. 2014. Neonatal gnotobiotic pig model of human enterovirus 71 infection and associated immune responses. Emerging Microbes and Infections 3, e35 (doi:10.1038/emi.2014.35).

Liu F, Wen K, Li G, Yang X, Kocher J, Bui T, Jones D, Vonck M, Pelzer K, Yuan L. 2014. Dual functions of Lactobacillus acidophilus NCFMTM™ in protection against rotavirus diarrhea ingnotobiotic pigs vaccinated with a human rotavirus vaccine. J Pediatr Gastroenterol Nutr. 58: 171-178 (doi:10.1097/MPG.0000000000000197).

Yuan L, Wen K, Liu F, Li G. 2013. Dose Effects of LAB on Modulation of Rotavirus Vaccine Induced Immune Responses. In Lactic Acid Bacteria - R & D for Food, Health and Livestock Purposes. Dr. J. Marcelino Kongo (Ed.), ISBN: 978-953-51-0955-6, InTech, p263-280. Available from:

Liu F, Li G, Wen K, Wu S, Zhang Y, Bui T, Yang X, Kocher J, Sun J, Jortner B, Yuan L. 2013. Lactobacillus rhamnosus GG on rotavirus induced injury of ileal epithelium in gnotobiotic pigs. J Pediatr Gastroenterol Nutr. 57: 750-758 (doi: 10.1097/MPG.0b013e3182a356e1).

Wentzel JF, Yuan L, Rao S, van Dijk A, O’Neill HG. 2013. Consensus sequence determination and elucidation of the evolutionary history of a rotavirus Wa variant reveal a close relationship to various Wa variants derived from the original Wa strain. Infect Genet Evol 20: 276-283 (doi:10.1016/j.meegid.2013.09.007).

Wu S, Yuan L, Zhang Y, Liu F, Li G, Wen K, Kocher J, Yang X, Sun J. 2013. Probiotic LGG mono-association suppresses virus-induced autophagy in gnotobiotic pig intestine. Gut Pathog. 5:22-28.

Bui T, Kocher J, Li Y, Wen K, Li G, Liu F, Yang X, LeRoith T, Tan M, Xia M, Zhong W, Jiang X, Yuan L. 2013. Median infectious dose of human norovirus GII.4 in gnotobiotic pigs is decreased by simvastatin treatment and increased by age. J. Gen Virol. 94:2005-2016.

Wen K, Li G, Yang X, Bui T, Bai M, Liu F, Kocher J, Yuan L. 2012. CD4+CD25-FoxP3+ regulatory cells are the predominant responding regulatory T cells after human rotavirus infection or vaccination in gnotobiotic pigs. Immunology. 137:160-171.

Azevedo MSP, Zhang W, Wen K, Gonzalez AM, Saif LJ, Yousef AE, Yuan L. Lactobacillus acidophilus and L. reuteri modulate cytokine responses in gnotobiotic pigs infected with human rotavirus. Benef Microbes 2012. 3, 33-42 (doi: 10.3920/BM2011.0041).

Wen K, Bui T, Li G, Liu F, Li Y, Kocher J, Yuan L.  Characterization of immune modulating functions of γδ T cell subsets in a gnotobiotic pig model of human rotavirus infection. Comp Immunol Microbiol Infect Dis. 2012. (Epub ahead of print) (doi:10.1016/j.cimid.2012.01.010) NIHMS353105.

Wen K, Li G, Bui T, Liu F, Li Y, Kocher J, Lin L, Yang X, Yuan L  High dose and low dose Lactobacilli acidophilus exerted opposite immune modulating effects on T cell immune responses induced by an oral human rotavirus vaccine in gnotobiotic pigs. Vaccine 2012. 30:1198– 1207 (doi:10.1016/j.vaccine.2011.11.107). NIHMS91341528.

Wen K, Li G, Zhang W, Azevedo MSP, Saif LJ, Liu F, Bui T, Yousef A, Yuan L. Development of γδ T cell subset responses in gnotobiotic pigs infected with human rotaviruses and colonized with probiotic lactobacilli. Vet Immunol Immunopathol. 2011; 141:267-27 (doi: 10.1016/j.vetimm.2011.03.016).

Liu F, Li G, Wen K, Bui T, Cao D, Zhang Y, Yuan L. Porcine small intestinal epithelial cell line (IPEC-J2) of rotavirus infection as a new model for study of innate immune responses to rotaviruses and probiotics. Viral Immunol. 2010;23:135-149 (doi: 10.1089=vim.2009.0088). PMC2883522.

Azevedo MS, Gonzalez AM, Yuan L., Jeong KJ, Iosef C, Nguyen TV, Lovgren-Bengtsson K, Morein B, Saif L. 2010. Oral versus intranasal prime/boost regimen using attenuated HRV or 2/6VLP with ISCOM influences protection and antibody secreting cell responses to rotavirus in a neonatal gnotobiotic pig model. Clin Vaccine Immunol 17:420-8, (doi:10.1128/CVI.00395-09). PMC2837955.

Yuan L, Honma S, Kim I, Kapikian AZ, Hoshino Y. Resistance to rotavirus infection in adult volunteers challenged with a virulent G1P1A[8] virus correlated with serum IgG antibodies to homotypic VP7 and VP4. J Infect Dis 2009;200:1443-1451 (doi: 10.1086/606116). PMC2760642.

Wen K, Azevedo MSP, Gonzalez AM, Zhang W, Saif LJ, Li G, Yousef AE, Yuan L. Toll-like receptor and innate cytokine responses induced by lactobacilli colonization and human rotavirus infection in gnotobiotic pigs. Vet Immunol Immunopathol. 2009;127:304-315 (doi:10.1016/j.vetimm.2008.10.322). PMC2653198.

Zhang, W, Azevedo, MSP, Wen K, Gonzalez AM, Saif LJ, Li G, Yousef AE, Yuan L. Probiotic Lactobacillus acidophilus enhances the immunogenicity of an oral rotavirus vaccine in gnotobiotic pigs. Vaccine 2008;26:3655-3661 (doi:10.1016/j.vaccine.2008.04.070). PMC2491559.

Yuan L, Wen K, Azevedo, SP, Gonzalez AM, Zhang W, Saif LJ. Virus-specific intestinal IFN-γ producing T cell responses induced by human rotavirus infection and vaccines are correlated with protection against rotavirus diarrhea in gnotobiotic pigs. Vaccine 2008;26:3322-3331 (doi: 10.1016/j.vaccine 2008.03.085). PMC2574896.

Zhang W, Azevedo MSP, Gonzalez AM, Saif LJ, Nguyen T, Wen K, Yousef AE, Yuan L. Influence of probiotic lactobacilli colonization on neonatal B cell responses in a gnotobiotic pig model of human rotavirus infection and disease. Vet Immunol Immunopathol. 2007;122:175-181 (doi:10.1016/j.vetimm.2007.10.003). PMC2268606.

Zhang W, Wen K, Azevedo, MSP, Gonzalez AM, Saif LJ, Yousef AE, Li G, and Yuan L. Lactic acid bacterial colonization and human rotavirus infection influence distribution and frequencies of monocytes/macrophages and dendritic cells in neonatal gnotobiotic pigs. Vet Immunol Immunopathol. 2008;121:222-31 (doi:10.1016/j.vetimm.2007.10.001). PMC2268605.

Nguyen T, Yuan L, Azevedo MSP, Jeong K, Gonzalez AM, Saif LJ. Transfer of maternal cytokines to suckling piglets: in vivo and in vitro models with implications for immunomodulation of neonatal immunity. Vet Immunol Immunopath. 2007;117: 236-48.

Nguyen T, Yuan L, Azevedo MSP, Jeong K, Gonzalez AM, Iosef C, Lovgren-Bengtsson K, Morein B, Lewis P, Saif LJ. High titer circulating maternal antibodies suppress effector and memory B cell responses induced by attenuated rotavirus priming and a rotavirus-like particle ISCOM boosting vaccine regimen. Clin Vaccine Immunol. 2006;13:475-85.

Nguyen T, Yuan L, Azevedo MSP, Jeong K, Gonzalez AM, Iosef C, Lovgren-Bengtsson K, Morein B, Lewis P, Saif LJ. Low titer maternal antibodies can both enhance and suppress B cell responses to a combined live attenuated human rotavirus and VLP-ISCOM vaccine. Vaccine 2006;24:2302-2316.

Yuan L, Stevenson G, Saif LJ. Rotavirus and Reovirus. In: Diseases of Swine. 9th Edition. Edited by Zimmerman, J. J., et al., Iowa State University Press, Ames, Iowa. 2006; pp.435-454.

Azevedo MSP, Yuan L, Pouly S, Gonzalez A, Jeong K, Nguyen T, and Saif LJ. Cytokine responses in gnotobiotic pigs after infection with virulent or attenuated human rotavirus. J. Virol. 2006;80:372-382.

Azevedo MSP, Yuan L, Jeong K, Gonzalez A, Nguyen T, Pouly S, Gochnauer M, Zhang W, Azevedo A, Saif LJ. Viremia and nasal and rectal shedding of rotavirus in gnotobiotic pigs inoculated with Wa human rotavirus. J. Virol. 2005;79:5428-5436.

Yuan L, Azevedo MSP, Gonzalez A, Jeong K, Nguyen T, Iosef C, Lewis P, Herrmann J, Saif LJ. Mucosal and systemic antibody responses and protection induced by a prime/boost rotavirus-DNA vaccine in a gnotobiotic pig model. Vaccine 2005;23:3925-3936.

Yuan L, Ishida SI, Honma S, Patton JT, Hodgins D, Kapikian A, Hoshino Y. Homotypic and heterotypic serum isotype-specific antibody responses to rotavirus proteins NSP4, VP4, VP6 and VP7 in infants who received selected live oral rotavirus vaccines. J. Infec. Dis. 2004;189:1833-1845.

Yuan L, Honma S, Ishida SI, Yan XY, Kapikian AZ, Hoshino Y. Species-specific but not genotype-specific primary and secondary isotype-specific NSP4 antibody responses in gnotobiotic calves and piglets infected with homologous host bovine (NSP4[A]) or porcine (NSP4[B]) rotavirus. Virology 2004;330:92-104.

Azevedo MSP, Yuan L, Iosef C, Chang KO, Nguyen TV, Kim Y, Saif LJ. Magnitude of serum and intestinal antibody responses induced by sequential replicating and non-replicating rotavirus vaccines in gnotobiotic pigs and correlation with protection. Clin Diag Lab Immunol 2004;11:12-20.

Hodgins DC, Yuan L, Parreno V, Corbeil LB, Saif LJ. Mucosal Veterinary Vaccines. In: Mucosal Immunology. Third Edition. J Mestecky, J Bienenstock, ML Lamm, L Mayer, JR McGhee and W Strober, Eds. Academic Press, San Diego. 2004; pp.1085-1107.

Yuan L, Saif LJ. Rotavirus-like particle vaccines evaluated in a pig model of human rotavirus diarrhea and in cattle. In: Perspectives in Medical Virology: Viral Gastroenteritis, Edited by A. J. Zuckerman and L. K. Mushahwar. Elsevier Sciences, Netherlands. 2003; pp.357-368.

Yuan L, Saif LJ. Induction of mucosal immune responses and protection against enteric viruses: rotavirus infection of gnotobiotic pigs as a model. Vet Immunol Immunopathol. 2002;87:147-60. Review.

Yuan L, Saif LJ. Mucosal B-cell memory to enteric viruses. Mucosal Immunology Update 2001; September:3-6. Review.

Qian Y, Yuan L. Rotavirus Vaccines. In: A New Generation of Vaccine, Edited by Z. M. Li. Higher Education Press, Beijing. 2001; pp. 475-86. [Chinese].

Yuan L, Geyer A, Saif LJ. Short-term immunoglobulin A B-cell memory resides in intestinal lymphoid tissues but not in bone marrow of gnotobiotic pigs inoculated with Wa human rotavirus. Immunology. 2001;103:188-98.

Yuan L, Iosef C, Azevedo MS, Kim Y, Qian Y, Geyer A, Nguyen TV, Chang KO, Saif LJ. Protective immunity and antibody-secreting cell responses elicited by combined oral attenuated Wa human rotavirus and intranasal Wa 2/6-VLPs with mutant Escherichia coli heat-labile toxin in gnotobiotic pigs. J Virol. 2001;75:9229-38.

Yuan L, Geyer A, Hodgins DC, Fan Z, Qian Y, Chang KO, Crawford SE, Parreno V, Ward LA, Estes MK, Conner ME, Saif LJ. Intranasal administration of 2/6-rotavirus-like particles with mutant Escherichia coli heat-labile toxin (LT-R192G) induces antibody-secreting cell responses but not protective immunity in gnotobiotic pigs. J Virol. 2000;74:8843-53.

Yuan L, Kang SY, Ward LA, To TL, Saif LJ. Antibody-secreting cell responses and protective immunity assessed in gnotobiotic pigs inoculated orally or intramuscularly with inactivated human rotavirus. J Virol. 1998;72:330-8.

Yuan L, Ward LA, Rosen BI, To TL, Saif LJ. Systematic and intestinal antibody-secreting cell responses and correlates of protective immunity to human rotavirus in a gnotobiotic pig model of disease. J Virol. 1996;70:3075-83.