BVSc, MVSc, PhD, Diplomate, ACVM
Department of Biomedical Sciences & Pathobiology
1976ECFVGAmerican Veterinary Medical Association Licensed to practice veterinary medicine & surgery in the states of Washington & Oregon
1974Ph.D., Molecular BiologyOregon State UniversityCorvallis, Oregon
1968M.V.Sc., Veterinary MicrobiologyUniversity of Agricultral SciencesBangalore, India
1966B.V.Sc., Veterinary Medicine and SurgeryUniversity of Agriculture SciencesBangalore, India
1978Diplomate, American College of Veterinary Microbiologists
- Targeted drug delivery for intracellular pathogens - In the last few decades, the emergence of drug resistant strains of Salmonella sp., Brucella sp., Mycobacterium sp., etc. representing intracellular bacterial pathogens is manifesting into a global health problem thereby raising new pharmaceutical challenges and necessitating the development of an efficient and cost effective novel agent. My laboratory is aiming to achieve site specific targeted drug delivery with increased bioavailability of antimicrobials/drugs using nanoparticles as carriers. Our ultimate goal is to design and develop novel nanoparticle based drugs by incorporating biological, engineering and chemical manufacturing principles for therapeutic application of chronic infectious diseases i.e., tuberculosis, brucellosis and salmonellosis. In addition, I also believe that development of nanoparticle based targeted drug delivery system may provide new therapeutic opportunities for the use of vast array of unstable, toxic, and insoluble antimicrobials (sitting on the shelves of drug companies), with minimum or no deleterious side effects.
- Development of vaccines against bioterrorism agents - My main interests are in understanding the host-parasite relationships, pathogenesis, virulence and their applications in vaccine development. At present my research focuses mainly on Brucella. My research effort is geared towards generation and testing of recombinant B. abortus RB51 vaccines against Anthrax, Tuberculosis, Paratuberculosis, Rift Valley Fever and Neosporosis.Microarray development to study host/pathogen interactions.
- Bacteriophage based remediation of food borne Salmonella in poultry - In the last six years, I initiated research to investigate the potential of phages as remedial agents in poultry. The entire chromosomal sequence (86.165kb) of one broad host range Salmonella species-specific phage called Felix 01has been determined. This was done to explore using such long-term surviving phages as therapeutic and or bioremedial agent against drug resistant Salmonella. Exploring bioremediation processes to treat regulated medical waste.
- Enterotoxigenic E. coli - My early efforts were focused on the isolation, purification and characterization of Heat-Stable Enterotoxin (STa). We were the first to show that bovine enterotoxigenic E.coli (ETEC) produced an 18 amino acid peptide. We also demonstrated by amino acid sequencing that bovine STa was similar, if not identical, to an 18-19 amino-acid peptide produced by human ETEC.
- Molecular Immunology - Characterization and quantitation of cytokine mRNA expression in lymphocytes from mice.
- Vaccines & aging - effect of aging on immune response
2003-presentProfessorVirginia TechBlacksburg, VA
1990-2003Associate ProfessorVirginia TechBlacksburg, VA
1984-1990Assistant ProfessorVirginia TechBlacksburg, VA
1979-1984Assistant ProfessorWashington State UniversityPullman, WA
1974-1975InternVeterinary ClinicCentralia, WA
1968-1970Veterinary SurgeonCivil Veterinary Hospital InstructorUASBangalore, India
- American Society for Microbiology (1973-present)
- American College of Veterinary Microbiologists (1978-present)
- American Association of Veterinary Laboratory Diagnosticians (1979-present)
- Conference of Research Workers in Animal Diseases (1982-present)
- Seleem MN, Boyle SM, Sriranganathan N. Brucella: A pathogen without classic virulence genes. Vet Microbiol. 2007 Nov 29.
- High KP, Prasad R, Marion CR, Schurig GG, Boyle SM, Sriranganathan N. Outcome and immune responses after Brucella abortus infection in young adult and aged mice.Biogerontology. 2007 Oct;8(5):583-93.
- Ramamoorthy S, Sanakkayala N, Vemulapalli R, Jain N, Lindsay DS, Schurig GS, Boyle SM, Sriranganathan N. Prevention of vertical transmission of Neospora caninum in C57BL/6 mice vaccinated with Brucella abortus strain RB51 expressing N. caninum protective antigens. Int J Parasitol. 2007 Nov;37(13):1531-8
- Contreras-Rodriguez A, Seleem MN, Schurig GG, Sriranganathan N, Boyle SM, Lopez-Merino A. Cloning, expression and characterization of immunogenic aminopeptidase N from Brucella melitensis. FEMS Immunol Med Microbiol. 2006 Nov;48(2):252-6.
- Seleem MN, Ali M, Boyle SM, Mukhopadhyay B, Witonsky SG, Schurig GG, Sriranganathan N. Establishment of a gene expression system in Ochrobactrum anthropi. Appl Environ Microbiol. 2006 Oct;72(10):6833-6.
- Honko AN, Sriranganathan N, Lees CJ, Mizel SB. Flagellin is an effective adjuvant for immunization against lethal respiratory challenge with Yersinia pestis. Infect Immun. 2006 Feb;74(2):1113-20.
- Vemulapalli TH, Vemulapalli R, Schurig GG, Boyle SM, Sriranganathan N. Role in virulence of a Brucella abortus protein exhibiting lectin-like activity. Infect Immun. 2006 Jan;74(1):183-91.
- Bandara AB, Sriranganathan N, Schurig GG, Boyle SM. Carboxyl-terminal protease regulates Brucella suis morphology in culture and persistence in macrophages and mice. J Bacteriol. 2005 Aug;187(16):5767-75.
- Whichard JM, Sriranganathan N, Pierson FW. Suppression of Salmonella growth by wild-type and large-plaque variants of bacteriophage Felix O1 in liquid culture and on chicken frankfurters. J Food Prot. 2003 Feb;66(2):220-5.
- Schurig GG, Sriranganathan N, Corbel MJ. Brucellosis vaccines: past, present and future. Vet Microbiol. 2002 Dec 20;90(1-4):479-96. Review.
- Zhi-Jun Y, Sriranganathan N, Vaught T, Arastu SK, Ahmed SA. A dye-based lymphocyte proliferation assay that permits multiple immunological analyses: mRNA, cytogenetic, apoptosis, and immunophenotyping studies. J Immunol Methods. 1997 Dec 15;210(1):25-39.
- Schurig GG, Roop RM 2nd, Bagchi T, Boyle S, Buhrman D, Sriranganathan N. Biological properties of RB51; a stable rough strain of Brucella abortus. Vet Microbiol. 1991 Jul;28(2):171-88.
- Sriranganathan N, Boyle SM, Schurig G, Misra H. Superoxide dismutases of virulent and avirulent strains of Brucella abortus. Vet Microbiol. 1991 Feb 15;26(4):359-66.
- Sriranganathan N, Burger D. Heat-stable (STa) enterotoxin of enterotoxigenic Escherichia coli: binding of the enterotoxin to coagulated milk and casein.Vet Microbiol. 1987 Feb;13(2):167-78.
- Sriranganathan N, Seidler RJ, Sandine WE, Elliker PR. Cytological and deoxyribonucleic acid-deoxyribonucleic acid hybridization studies on lactobacillus isolates from San Francisco sourdough. Appl Microbiol. 1973 Mar;25(3):461-70.