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DBSP Research Faculty

Mohamed N. Seleem

Mohamed N. Seleem, BVSc, MS, PhD

Postdoctoral Associate
Department of Biomedical Sciences & Pathobiology
Mentor: Dr. Nammalwar Sriranganathan

e-mail: naguieb@vt.edu


Education

2006 PhD, Molecular Microbiology
VA-MD Regional College of Veterinary Medicine
Virginia Tech, USA
2000 MSc, Zoonoses
College of Veterinary Medicine
Assiut University, Egypt
1995 B.V.Sc
College of Veterinary Medicine
Assiut University, Egypt

Research Project Involvement

Targeted drug delivery for intracellular pathogens
Intracellular pathogens like Mycobacterium, Brucella, Salmonella and Listeria have developed various mechanisms to evade host defenses, and they can establish chronic infections. Treatment and eradication are difficult since infections are localized within phagocytic cells and most antibiotics, although highly active in-vitro, do not actively pass through cellular membranes. My research is focused on site-specific targeted drug delivery with increased bioavailability of antimicrobials/drugs using nanoparticles as carriers. We are using Salmonella and Brucella as model of intracellular pathogens.


Professional Experience

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2007-Present Postdoctoral Research Associate
The Institute for Critical Technology and Applied Science
Virginia Tech, USA
2006-2007 Postdoctoral Research Associate
College of Veterinary Medicine Cornell University, USA
2003-2006 Teaching and Research Assistant
VA-MD Regional College of Veterinary Medicine
Virginia Tech, USA
2001-2003 Research Scientist
VA-MD Regional College of Veterinary Medicine
Virginia Tech, USA
1995-2001 Lecturer and Research Scientist
College of Veterinary Medicine
Assiut University, Egypt

Awards

  • Phi Zeta Manuscript Award in the Basic Sciences, Virginia, USA, January 2008.
  • International Distinguished Dissertation Award-Biological and life Sciences. The Council of Graduate Schools (Nominated-Final Selection), Washington, DC, USA, October 2007.
  • Outstanding Dissertation Award-Sciences and Engineering, Virginia Tech, USA, March 2007.
  • Best paper and research excellence the 23rd International Research Symposium of the College of Veterinary Medicine, Assiut, Egypt, December 10-12, 2006.
  • Virginia Bioinformatics Institute (VBI) Scholarship Award, USA, April 26th, 2006.
  • Best presentation award and Research Excellence in 16th Annual Research Symposium, Virginia-Maryland Regional College of Veterinary Medicine, USA, June 17th 2004.
  • Best presentation award and Research excellence 56th Animal Disease Research Workers in the Southern States, USA, April 6th 2005.
  • The Rick Ciordia-T. Bonner Steward Award For Excellence in Animal Disease Research, USA, April 2005.
  • Outstanding Recognition Award, SEED Pharmaceutical company, Egypt, 1995.
  • Outstanding Recognition Award, HOFMA Pharmaceutical company, Egypt, 1995.

Selected Publications

2008

  1. Seleem, M. N., N. Jain, H. Alqublan, R. Vemulapalli, S. M. Boyle, and N. Sriranganathan. 2008. Activity of native vs. synthetic promoters in Brucella. DOI:10.1111/j.1574-6968.2008.01358 FEMS Microbiol Lett.
  2. Rajasekaran, P., M. Seleem, A. Contreras, N. Sriranganathan and S. Boyle, A leucine auxotroph of Brucella abortus strain RB51 vaccine as an environmentally safe vector for plasmid maintenance and antigen expression. doi:10.1128/AEM.01511-08, “Applied and Environmental Microbiology”.
  3. N. Pothayee, M. L. Vadala, A. Ranjan, N. Jain, M. Seleem, N. Sriranganathan and J. S. Riffle. 2008. Aminoglycoside-Ionopolymeric nanoplexes for targeting intracellular bacterial pathogens. Polymer Preprints,49(2), 1036
  4. Seleem, M.N., M. Ali, S.M. Boyle and N. Sriranganathan. 2008. Reporter genes for real-time in vivo monitoring of Ochrobactrum anthropi infection. 286:1;124-9 “FEMS Microbiological letters”
  5. Seleem, M.N., M. Ali, S.M. Boyle and N. Sriranganathan. 2008. Vectors for enhanced gene expression and protein purification in Salmonella. 421: 95-8. “GENE”
  6. I. Sandal, S., Seleem, M.N., Elswaifi, N. Sriranganathan and T. Inzana. 2008. Efficient expression vectors for Histophilus somnus. 74;106-9. Microbiological Methods.
  7. Seleem, M.N., P. Rajasekaran, M. Ali, S.M. Boyle and N. Sriranganathan. 2008. Simple Method for Transformation of Ochrobactrum anthropi. 24;10: 2111-4 “World Journal of Microbiology and Biotechnology”
  8. Seleem, M.N., S.M. Boyle and N. Sriranganathan. Brucella; a pathogen without classical virulence genes. 2008:25;129:1-14., “Veterinary Microbiology”.

2006-2007

  1. Seleem, M.N., M. Ali, M. Abd Alazim, S. Boyle and N. Sriranganathan. 2007, Enhanced expression, detection and purification of recombinant protein using tandem fusion tags RNA stem loop. 75:1385-1392: "Applied Microbiology and Biotechnology.
  2. Seleem, M.N., M. Ali, M. Abd Alazim, S. Boyle and N. Sriranganathan. 2007. High-level heterologous gene expression in Ochrobactrum anthropi using an A-rich UP element. 73(5):1123-7: “Applied Microbiology and Biotechnology”.
  3. Seleem, M.N., M. ALI, S.M. Boyle, and N. Sriranganathan. Establishment of gene expression system in Ochrobactrum species. 2006, p. 6833-6836, Vol. 72, No. 10 “Applied and Environmental Microbiology”.
  4. A, Contreras, Seleem, M.N., S. M. Boyle, G. Schurig, N. Sriranganathan and A. Lopez-merino. Cloning, expression and characterization of immunogenic aminopeptidase N from Brucella melitensis. 2006 Nov;48(2):252-6 FEMS Immunology and Medical Microbiology.
  5. A, Bandara, Seleem, M.N., C. Jordan, D. Lindsay, G. Schurig, and N. Sriranganathan. Brucella abortus Strain RB51 can be used to Express Potentially Protective Antigens of Toxoplasma gondii., 53(S1), 2006 pp. S166–8. J. Eukaryot. Microbiol.

Book Chapter

Brucella Chapter, Genome Mapping in Animals and Microbes, Volume 8- Animal Associated Microbes (Springer Jan 3rd -2009).