Roster  DBSP Departments VA-MD Vet Med

DBSP Research Faculty

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Guohua Li, PhD

Research Scientist, Molecular Virology
Department of Biomedical Sciences & Pathobiology

e-mail: lguohua@vt.edu


2005-2007 Postdoctoral, Molecular Biology
Ohio State University
Columbus, Ohio
1998-2001 PhD, Molecular Biology
Chinese Academy of Medical Sciences & Peking Union Medical College
1993-1996 MS, Molecular Biology
Capital Institute of Pediatrics
1986-1991 BS, Clinical Medicine
Jiangxi Medical College

Research Project Involvement

Project Name: Construction and evaluation of reverse genetics systems of human and porcine rotaviruses

Project Description: This project is supported by CVM Internal Research Competition (IRC). The goal of this project is to establish a plasmid-based reverse genetics system for generating infectious rotavirus particles. This system will be based on the recombinant vaccinia virus T7 RNA polymerase-driven procedure to supply artificial authentic viral mRNA which can be used for replication of double-stranded (ds) RNA and translation of rotavirus protein. The cDNA segments representing 11 genes of rotavirus will be cloned into individual plasmid vector pX8dT. The infection of rDIs-T7pol and transfection of recombinant plasmids containing the cDNAs of 11 rotavirus segments respectively will allow transcription of the positive-strand RNAs of rotavirus genome and translation of all rotavirus proteins. Our hypothesis is that the presentation of all rotavirus proteins and positive-strand RNA segments in cells can initiate the replication and packaging of rotavirus. Reverse genetics system will provide a tool for studies of pathogenesis and immunogenicity of rotaviruses and for development of effective and safe rotavirus vaccines for humans and animals.

Professional Experience

2007-Current Research Scientist, Virginia Polytechnic Institute and State University
2005-2007 Post-doctoral Researcher, The Ohio State University, Advisor Dr. Linda J. Saif
2004-2005 Research Assistant, Institute of Molecular Biology, the University of Hong Kong, Hong Kong
2001-2004 Research Associate, Capital Institute of Pediatrics, China, Advisor Yuan Qian
1996-1998 Research Assistant, Capital Institute of Pediatrics, China, Advisor Yuan Qian
1991-1993 Physician, Hospital of Shanggao County, Jiangxi Province, China

Professional Memberships

  • American Society for Virology