VM8054 Veterinary Histology

Technical Notes

Author: Dr. Thomas Caceci

This project began as a series of histology lab notes in 1982, when I was teaching at Texas A&M. Inexorably, in accordance with the Law Of Academic Paperwork, over the years the revisions, additions, and expansions increased the size of the package from its original slim 20-odd pages to a bulging 165 page handout, distributed to the class each year at considerable expense.


The first versions were written on an IBM Selectric II, a device that represents the absolute zenith of typewriter technology: no better purely mechanical machine has ever been made, anywhere, by anyone, period. But it was obsolete even in 1982, and it's a museum piece now, as dated as coal oil lamps and gentlemen's spats. Beginning with the third edition on paper, progressively more up-to-date "word processing" programs were used.


In the mid 1980's I made the wrenching transition from my reliable and predictable Selectric to the quirks and idiosyncrasies of Wordstar 3.3, at that time the cutting edge of "word processing" programs. This was a big leap for someone who wrote his Ph.D. dissertation—all five drafts of it—on a manual Smith-Corona portable typewriter dating to the Korean War. No one who has ever learned to use "word processor" software would ever willingly go back to using a typewriter, but there are times when I do think fondly of my old blue IBM dinosaur and wonder where it is.

The first "word processing" of these notes was done around 1984 or 85, so early in the game that I wasn't even using a computer running under DOS. Bill Gates hadn't yet achieved a lock on the personal computer market, and my first computer was a Kaypro II running some now forgotten CPM software, and the Wordstar 3.0 version for it. Nor did there exist such a thing as a scanner with optical character recogntion. I had to type the whole damned thing over again.

Despite my misgivings I survived that initial move to "word processing." That phrase still bothers me, but pushing electrons around seems to merit a different term than "writing," and after all, it's no more technocratic than "typewriting" was in, oh, 1895. I draw the line, however, at the use of "keyboard" as a verb: as in, "I'm going to keyboard that document." That much of my vocabulary standards remains intact.


By the time I came to Virginia Tech in 1987, the notes I'd accrued (which were still, of course, distributed on paper) needed revision and expansion again. I had moved up to Wordstar 5.0, which was used to produce the third edition. Along the way I'd made the change from CPM to DOS and was, like 95% of other computer users, helping to make Mr. Gates the richest man in America.


The fourth edition of the lab manual was written in Wordstar 7.0. Between the time when I started using Wordstar 5.0 and then, I'd actually purchased version 6.0, but hadn't got around to installing it. I never did. On the very day that I said to myself, "OK, now I have enough time to install that new Wordstar," I received a notice in the mail about Version 7.0! So I ordered that, and version 6.0 still sits moldering in its box. Perhaps someday in the future a collector of rare early software will be delighted to get it at my estate auction, still pristine in its shrink wrap.


The 5th edition of the manual (still on paper) was the transitional one. By the time I began to write it in early 1995, the world was shifting to Microsoft Word. Word has the distinct advantage of producing documents that can be read by both PCs and Mac machines, a matter of considerable importance. If there was ever a version of Wordstar for Macs, I never heard about it.


I was comforted in my affliction to know that between 1987 and 1995 I had managed to entirely avoid the use of Word Perfect in any form. Word Perfect was the ne plus ultra of "word processors" at one time, and had eclipsed the earlier and much better Wordstar entirely. I always hated it and was happy to see it die the death it deserved under the onslaught of Microsoft Word. Nevertheless, just as every now and then a straggler from the Imperial Japanese Army emerges from the Phillippine jungles, there are still some institutions which use Word Perfect. A few days ago I received an attachment in my e-mail written with it and was completely unable to read it.


Unfortunately I was still stuck with the 5th Edition in Wordstar 7.0, and I had to convert it to Word so that it could be re-worked into HTML code. It didn't help things that by that time I was one of perhaps four people left in the USA still using any form of Wordstar at all. However, the clever programmers at Mastersoft had come up with Word for Word, a very slick conversion program that allowed me to take my Wordstar document and change it over—more or less perfectly—to anything else. Mercifully I wasn't faced with the horrible prospect of re-typing all 165 pages of my dense, single-spaced, turgid prose as a new document. Word for Word took all 500 kilobytes of that 5th Edition and created a Word document in less than ten minutes.

In 1994-95, the surging growth of the World Wide Web made it plain that the days of paper handouts were numbered. In addition to the enormous bulk, the costs were rising and seriously pinching us in a time of budget cuts. The College was spending well over $40,000 per year for class handouts, and of that total some $800-900 per year went just to Histology. Obviously something had to be done. The answer was to devise a paperless version of the manual.

In the final analysis I decided I had to "go digital," and that I had to use something that was "cross platform" (the VMRCVM was officially a Mac-based institution, though I fought them fiercely all the way) and could be accessed by anyone. Given the wide variety of "word processing" programs then in use, more or less by accident I stumbled into the idea of writing the whole thing for the then-new "World Wide Web." That meant I had to teach myself to write Hypertext Markup Language (HTML), the programming code used for the WWW.


In its purest form, HTML language is essentially straight ASCII text, and it can be written with any "word processing" software if you know the codes and tags it uses. I knew some basic ones in 1995 but eager software developers were turning out new versions of HTML faster than I could learn them. Furthermore, Wordstar was a wonderful "word processing" program but it wasn't especially good for writing plain ASCII text and HTML code. Microsoft Word is a clumsy HTML editor but it's better for the purpose than Wordstar, so as hard as it was for me to abandon my painfully-learned DOS 3.1 and Wordstar, and delve into the mysteries of Windows and Word, I had to do it.

The College faculty was being asked to devise ways to deliver their information to students at lower cost, as well. Since I wanted to include many more illustrations, and to use color if possible, writing the revisions as HTML documents and creating a self-contained WWW site on a CD was the logical next step. The problem was conversion of the MS-Word version to the HTML format. This was easily solved by acquiring a copy of an add-on program for MS-Word, Internet Assistant. Internet Assistant could read a file in early versions of Word and convert it to HTML; later versions of Word do it automatically. I was set for the emergence of the electronic manual, Version 1.0.

While it worked well for conversions, Internet Assistant was a first-generation HTML editor, so I used it only to convert the pre-existing text. All the new text, all of the original captions for the images, and all the notes and additional material, were prepared using HTML Writer, a very fine freeware program written by Kris Nosack at Brigham Young University. HTML Writer was a very early attempt at making a user-friendly HTML editor and has long since become obsolete as the WWW has grown and new versions of the HTML language have been developed.


Version 2.0 was edited and re-configured using Corel WebDesigner. Subsequent to that, all versions has been edited with Macromedia's Dreamweaver programs, starting with Dreamweaver 3.0 and currently (June 2005) Dreamweaver MX. Dreamweaver is pretty much the standard in HTML editors these days. It's very nearly as easy to use as HTML Writer was and far more powerful. In addition it has the virtue of being able to strip out of a document the hundreds of lines of WORD-specific code (read: "gibberish") that result when using Word to write HTML. The documents Dreamweaver produces are plain-vanilla HTML code, easy to interpret even in native format.


All the images of microscopic sections were taken specifically for the CDs, stored as .JPG files. Most of them were shot on an Olympus Vanox photomicroscope. Originally they were recorded on Kodak Ektachrome film and digitized using an Apple Macintosh computer, Photoshop 3.0 software, and a Microtek Scanmaker 1850S slide scanner; then edited with L-View and/or L-View Pro 3.0, for version 1.0. In Versions 1.5 and later editing was done with Photoshop 4.0, 5.0 and 7.0. A Polaroid SprintScan 35LE was used for images scanned from slides after Version 2.0.


In most cases, post-Version 1.5 updates include images that never existed on film, however. They were captured digitally on the Vanox after retrofitting it first with a Mideo Image Processing system and most recently with an Olympus DP-70 digital camera. The line drawings and old photographs were digitized with an Epson 1000C, Paperport 3100, or Paperport 9200 flatbed scanners. The editing and assembling and re-writing of the first electronic version was done on a Pionex 486-based PC machine; this old workhorse was put out to pasture long ago, replaced first by a (shudder) Power MacIntosh, which was replaced in its turn by a Dell OptiFlex GX1, a Dell Optoplex GX240, and finally a Dell Inspiron 5150 laptop.


Througout the process, though the equipment and (I hope) the final product has been upgraded significantly from where it was a decade ago, the goal has never changed: to present the topic as clearly and as well illustrated as I can. Since in that time I haven't been upgraded, only the user can decide whether this goal has been attained.

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VM8054 Veterinary Histology